Chaya Kalcheim

Professor

Our laboratory aims at elucidating basic processes taking place during embryonic development of the nervous and musculo-skeletal systems. We focus on four main subjects: first, the development of the peripheral nervous system (PNS) from the neural crest. Second, the cellular and molecular basis underlying the transition from the neural crest state (PNS) to the definitive roof plate of the CNS. Third, morphogens and mechanisms involved in muscle and vertebral development. Fourth, the molecular signals that mediate interactions between mesoderm and nervous system, found to be of significance for patterning and differentiation.Our lab has a rich academic record in avian embryology and molecular techniques that include in ovo microsurgery and grafting, targeted gene missexpression in embryos, in vitro explants and culture systems, imaging and RNAseq techniques.

Research and Projects

Introduction

We are currently interested in elucidating the mechanisms leading to the end of Neural Crest production and emigration and the concomitant transition into the Roof plate of the dorsal CNS domain.

In addition, we pursue experiments to understand the patterning of skeletal muscles and vertebrae, and the roles of morphogens such as Sonic hedgehog, etc in coordinating skeletal with neural development.

Our work focuses on avian embryos (chick and quail) and includes a variety of in vivo techniques, from tissue grafting to gene missexpression, in vitro explants, molecular profiling and imaging. We actively collaborate with researchers in the mammalian field to complement our knowledge using genetic models.

Project 1- The transition between neural crest and definitive roof plate.

Research on the development of the dorsal neural tube is particularly challenging. In this highly dynamic domain, a temporal transition occurs between early neural crest progenitors that undergo an epithelial-to-mesenchymal transition and exit the neural primordium, and the subsequent roof plate, a resident epithelial group of cells that constitutes the dorsal midline of the central nervous system. Among other functions, the roof plate behaves as an organizing center for the generation of dorsal interneurons. Despite extensive knowledge of the formation, emigration and migration of neural crest progenitors, little is known about the mechanisms leading to the end of neural crest production and the transition into a roof plate stage.

image from: Rekler, D. and Kalcheim, C. (2021). From Neural Crest to definitive Roof Plate: the dynamic behavior of the dorsal neural tube. Int. J. Mol. Sci.

Project 2-How does Sonic hedgehog induce motoneuron differentiation?

The sonic hedgehog (Shh) protein plays fundamental roles in the development of the neural tube (NT) and somites. Following neurulation, Shh secreted by the notochord (No) induces distinct ventral cell identities in the overlying NT by a mechanism that depends on relative concentrations and duration of exposure. Moreover, the activity of Shh continues beyond this stage to regulate cell proliferation, survival and differentiation. No-derived Shh is also involved in mesoderm patterning.

In our lab we investigate the mechanisms of action of Shh in neural and mesodermal development.

image from: Kahane, N. and Kalcheim, C (2020). Neural tube development depends on notochord-derived Sonic hedgehog released into the sclerotome. Development.

Funding sources

Our lab has been generously supported over the years by the Israel Science Foundation, DFG, IMRF, March of Dimes, ICRF and Familial Dysautonomia Foundation.

Publications

Rekler, D. and Kalcheim, C. (2022). Completion of neural crest cell production and emigration is regulated by retinoic-acid-dependent inhibition of BMP signaling. eLife.

In this paper we reveal a role for neural tube-derived Retinoic Acid in ending the neural crest production. Retinoic acid acts through BMP signaling to downregulate neural crest-specific genes and to stop cell emigration from the neural tube. While it is also important for aspects of the development of definitive Roof Plate, not all Roof Plate traits depend on retinoic acid, suggesting that the end of neural crest production and the formation of the Roof Plate are in part independently regulated.

Ofek, S, Wiszniak, S, Kagan, S, Tondl, M, Schwarz, Q, and Kalcheim, C. (2021). Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube. BMC Biol.

In this article we used RNA-seq to reveal differences in gene expression profile between Neural Crest and definitive Roof Plate cells. Based on these findings we proceed to show the importance of Notch signaling in the formation of the definitive Roof Plate of the spinal cord and highlight its role in the segregation between Roof Plate and dorsal interneurons.

Rekler, D. and Kalcheim, C. (2021). From Neural Crest to definitive Roof Plate: the dynamic behavior of the dorsal neural tube. Int. J. Mol. Sci.

A review highlighting current knowledge about the transition between Neural Crest and definitive Roof Plate phases.

Kahane, N. and Kalcheim, C (2020). Neural tube development depends on notochord-derived Sonic hedgehog released into the sclerotome. Development.

Recommended by Faculty Opinions (F1000).

In this article we reveal a role of the sclerotome as a site of Shh gradient, controlling development of mesodermal and neural progenitors.

Kumar, D, Nitzan, E. and Kalcheim, C (2019). YAP promotes neural crest emigration through interactions with BMP and Wnt activities. Cell Communication and Signaling.

Here we found a role of YAP signaling in Neural Crest EMT and examined its interaction with BMP/Wnt pathways.